Menu:

To obtain service, please call or email Martin-Protean.

Collision induced dissociation of peptide bonds in msms spectra

Instrumentation

System Suitability

System Sensitivity

Capabilities

Fragment mass assignments for spectra

Instrumentation

Nano LCMSn capability

Using a Waters nanoAcquity ultra pressure liquid chromatography system, in-line nanospray ionization, and a Thermo Deca XP+ ion trap mass spectrometer, our nano LCMSMS setup can give the customer robust peptide identification from samples containing 100 fmols of material.

Protein identification from msms spectra

Using the latest version of the Discoverer 1.0 (replaced Sequest) peptide and protein assignment software and our years of experience assigning peptide spectra, Martin-Protean can provide careful and thorough protein assigment from the peptide spectra generated from your samples.

Protein modification reagent synthesis and purification

MP facilities have a cold water plumbed chemical fume hood, Buchi R114 rotary evaporator with Welch pump, Mettler Toledo AT261 Delta Range analytical balance, Foxy 200 flash chromatography system, glassware oven, Chemglass glassware and 4C, -20C, -80C storage.

Protein biochemistry and molecular biology

MP facilities have Elga PureLab Prima & Ultra 18MOhm water purifier, Mettler Toledo PB3002-S/Fact balance, Accumet 25 pH conductivity meter, Beckman 530 UV/Vis spectrophotometer, two Eppendorf 5415D bench top centrifuges, Beckman Coulter Allegra 6KR clinical centrifuge, New Brunswick Scientific Innova 4200 incubator/shaker, MJResearch PTC-200 DNA engine thermocycler, BioRad E coli pulser, and protein and DNA electrophoresis equipment.

System Suitability

The data you need when you need it: our current ESI-LCMSn system suitability run.

System Sensitivity

The the sensitivity you need when you need it: our current ESI-LCMSn limit of detection, linear to 3 fmoles for peptide in standard data collection mode.

Capabilities

Martin-Protean has extensive experience in: